龙牙百合的植株再生与遗传转化

以龙牙百合鳞片叶切块为外植体，通过多种培养基的比较试验，得出MS 2，4-D 2mg／L 6-BA 0．2mg／L是诱导愈伤的最佳培养基，MS 6-BA 1mg／L NAA 0．05mg／L是不定芽诱导及伸长的适宜培养基。MS N_AA2mg／L是生根的最佳培养基。本试验已建立了适用于龙牙百合遗传转化的快速高频再生系统。用携带有几丁质酶基因和β—1、3葡聚糖酶基因的工程菌，通过农杆菌介导法和基因枪转化法转化龙牙百合，经PCR和点杂交检测证明外源基因已经整合到植物染色体中。同时对农杆菌介导法和基因枪法进行比较，发现农杆菌介导法的转化率为16．7％，基因枪法的转化率为50％，因此可能基因枪转化法更适于龙牙百合的遗传转化。     

引诱剂对柑桔小实蝇、瓜实蝇诱捕效果试验

用引诱剂甲基丁香酚、4-(P-醋酸基-苯基)2-丁酮(简称Cuelure)与这两种药的混合剂(简称Islon)对柑桔小实蝇和瓜实蝇(雄虫)进行诱捕试验,结果表明,国产与日本产的这两种引诱剂的效果无明显差异,日产的甲基丁香酚药效持久性稍长于国产的;cuelure与Islon对瓜实蝇的诱捕效果无明显差异;甲基丁香酚对柑秸小实蝇的诱捕效果稍高于Islon剂。推荐用Islon作检疫或监测实蝇的引诱剂。本文还对柑桔小实蝇和瓜实蝇的虫口变动与寄主之间的关系进行了观察。     

野百合鳞茎芽的诱导和增殖的初步研究

以野生百合的鳞茎为外植体,研究鳞茎的最佳消毒时间、诱导鳞茎芽的最佳部位、影响鳞茎芽诱导的最佳激素组合以及不同激素对鳞茎芽增殖的影响。结果表明:用75%酒精30 s+0.1%HgCl25 min消毒效果最好。鳞茎芽的诱导能力依次是:内层>中层>外层;基部>中部>上部。MS+NAA 0.5 mg/L+6-BA 1.5 mg/L对鳞茎芽的诱导比较好,MS+6-BA 0.8 mg/L+NAA 0.05 mg/L对鳞茎芽的增殖比较好。     

转Bt基因水稻对东亚飞蝗抗寒性的影响

为了探究转Bt基因水稻对东亚飞蝗抗寒性的影响,在室内条件下以转Bt基因水稻和非转基因水稻分别喂养东亚飞蝗3代,用过冷却点仪测定东亚飞蝗的过冷却点和冰点。结果表明,转Bt基因水稻饲养的东亚飞蝗的4龄、5龄幼虫、成虫（雌和雄）的过冷却点分别为-5.89、-7.59、-6.64和-6.79,与对照组无显著差异。     

一种准确简便的东方白鹤性别分子鉴定方法

东方白鹤为国家Ⅰ级重点保护动物，在自然条件下繁殖率很低，进行迁地保护及再引入措施对遗传多样性保护具有举足轻重的作用，成功地进行性别鉴定是其笼养人工繁殖的前提。本研究对4只东方白鹤，运用CHD基因的一对引物2550F／27181K进行了准确简便的性别鉴定，并对其序列结合其他5种远缘鸟类相应序列进行分析，证实了此种方法的科学性和可信度，分析了性别鉴定机理。这种安全、方便、准确的性别分子鉴定方法将有助于东方白鹤迁地保护及再引入措施的实施。通过分析可以看出，此种方法未来存在着广阔的应用前景。     

百合鳞片扦插繁殖的研究进展

鳞片繁殖是百合种球生产过程中的关键环节.综述了迄今为止有关鳞片扦插技术、影响鳞片扦插繁殖的因素和鳞片繁殖生理等方面的研究成果,并对该领域存在的问题进行了简析,对今后的研究重点进行了展望.     

Physiology Mechanism of Metabolisms in the Middle Scales of Lilium davidii var. unicolor Bulbs Stored at Low Temperature for Dormancy-Release

Changes in carbohydrate, phenols, free amino acids and endogenous hormones in middle scales of Lilium davidii var.unicolor bulbs during the 101 days storage at 2, 6 and 10℃ in moist sawdust were studied. The results indicated that as the storage time increased and the storage temperature delined, contents of starch and abscisic acid (ABA) decreased markedly, and the level of total soluble sugar, phenols, gibberellic acid (GA3) and indole acetic acid (IAA) increased sharply. In the first 34 days, the contents of the above substances changed most actively. Correlation analysis revealed that the increase in content of total soluble sugar and phenols was respectively caused by activation of amylase and phenylalanine ammonia-lyase (PAL). During the storage, the content of free amino acids decreased, and the level of arginine was the highest and changed most remarkably. According to the results of stepwise regression analysis and path analysis, we could primarily conclude that the metabolism of carbohydrates, phenols and free amino acids in the middle scales may be mainly regulated by endogenous GA3.     

Acupuncture treatment for idiopathic Horner's syndrome in a dog

A one-year-old female English Cocker Spaniel dog with idiopathic Horner''s Syndrome is described. The specific clinical signs in this specimen were miosis, ptosis, enophthalmos, and prolapsed nictitans for 2 days following sudden onset. According to history taking, ophthalmic, neurological, and radiological examination, the patient was diagnosed with idiopathic Horner''s syndrome. Manual acupuncture treatment was applied to the dog on local points two times in 2 days. The local acupoints were ST-4 (Di Chang) and GB-1 (Tong Zi Liao). The day after the initial acupuncture treatment, clinical signs related to idiopathic Horner''s syndrome had almost disappeared. The day after the second treatment, specific clinical signs were completely absent. During this period, the dog did not receive any orthodox treatment. Thus, it is suggested that manual acupuncture might be an effective therapy for idiopathic Horner''s syndrome.     

岷江百合天然群体的表型多样性

 以岷江百合(Lilium regale Wilson)的7个天然群体为研究对象,对其株高、花瓣长、叶片数、花朵数、叶片长和叶片宽等6个表型性状进行多样性分析。结果表明：岷江百合表型性状在群体间存在广泛变异,6个性状群体间的F值为4.878～34.915,达显著或极显著水平;群体内只有叶片长和叶片宽达极显著水平,其他4个性状均不显著。6个性状的平均表型分化系数(VST)为61.5%,群体间变异（26.2%）大于群体内变异（20.0%）,说明群体间变异是百合表型性状的主要变异来源。岷江百合表型性状与地理因子的相关分析表明：株高、花朵数和叶宽与纬度成显著正相关,而其它性状与地理因子的相关性均不显著。利用群体间欧氏距离进行的UPGMA聚类分析结果表明,7个岷江百合天然群体可以划分为两类,说明性状的表型特征并没有依地理距离而聚类。     

毛百合×有斑百合种间杂种的育成

以毛百合(Lilium dauricum)为母本、有斑百合(L.concolor var.prlchellum)为父本进行常规杂交,通过胚培养成功获得了种间远缘杂交种,对种问杂交亲和性、胚培养和杂种的形态特征进行了调查研究.结果表明:毛百合×有斑百合蒴果膨大率达46.1%,种子有胚率为1.30%,胚培养萌发率为15.2%;4个杂交后代生长旺盛,株高表现出较明显的超亲现象,其他性状呈趋中趋势,杂种植株的花色更偏向橙色.4个杂交后代虽然在花色上与亲本有一定差异,但其它性状均具有双亲的特征,从形态上能证明是真杂种.